Y. Komaragiri, R. H. Pires, S. Spiegler, H. T. Dau, D. Biedenweg, C. O. Salas, Md. F. Hossain, B. Fregin, S. Gross, M. Gellert, U. Lendeckel, C. Lillig, and O. Otto
Reactive oxygen species induce cell stiffening through lysosomal degradation and subsequent intracellular acidosis in myeloid precursor cells
Communications Physics 7:252 (2024)

C. Redwanz, R. H. Pires, D. Biedenweg, S. Gross, O. Otto, S. Könemann
Endothelin-1 influences mechanical properties and contractility of hiPSC derived cardiomyocytes resulting in diastolic dysfunction
Journal of Molecular and Cellular Cardiology 194: 105 – 117 (2024)

A. K. Yanamandra, J. Zhang, G. Montalvo, X. Zhou, D. Biedenweg, R. Zhao, S. Sharma, M. Hoth, F. Lautenschläger, O. Otto, A. del Campo, and B. Qu
PIEZO1-mediated mechanosensing governs NK-cell killing efficiency and infiltration in three-dimensional matrices
European Journal of Immunology 0:2350693 (2024)

M. Sampietro, V. Cassina, D. Salerino, F. Barbaglio, E. Buglione, C. A. Marrano, R. Campanile, L. Scarfò, D. Biedenweg, B. Fregin, M. Zamai, A. D. Torres, V. L. Cantarero, P. Ghia, O. Otto, F. Mantegazza, V. R. Caiolfa, and C. Scielzo
The Nanomechanical Properties of CLL Cells Are Linked to the Actin Cytoskeleton and Are a Potential Target of BTK Inhibitors
Hemasphere 7, e931 (2023)

M. Herbig, A. Jacobi, M. Wobus, H. Weidner, A. Mies, M. Kräter, O. Otto, C. Thiede, M. T. Weickert, K. S. Götze, M. Rauner, L. C. Hofbauer, M. Bornhäuser, J. Guck, M. Ader, U. Platzbecker, and E. Balaian
Machine learning assisted real-time deformability cytometry of CD34+ cells allows to identify patients with myelodysplastic syndromes
Scientific Reports, 12, 870 (2022)

Y. Komaragiri, M. H. Panhwar, B. Fregin, G. Jagirdar, C. Wolke, S. Spiegler, and O. Otto
Mechanical characterization of isolated mitochondria under conditions of oxidative stress
Biomicrofluidics 16, 064101 (2022)

V.A.S. Dabbiru, E. Manu, D. Biedenweg, P. Nestler, R. H. Pires, and O. Otto
Cell-surface contacts determine volume and mechanical properties of human embryonic kidney 293T cells
Cytoskeleton 80: 21 – 33 (2022)

B. Meesaragandla, Y. Komaragiri, R. Schlüter O. Otto, and M. Delcea
The impact of cell culture media on the interaction of biopolymer-functionalized gold nanoparticles with cells: mechanical and toxicological properties
Scientific Reports 12,16643 (2022)

B. Fregin, D. Biedenweg, and O. Otto
Interpretation of cell mechanical experiments in microfluidic systems depend on the choice of cellular shape descriptors
Biomicrofluidics 16, 024109 (2022)

J. Baumann, L. Sachs, O. Otto, I. Schoen, P. Nestler, C. Zaninetti, M. Kenny, R. Krantz, H. v. Eysmondt, J. Rodriguez, T. E. Schäffer, Z. Nagy, A. Greinacher, R. Palankar, and M. Bender
Reduced platelet forces underlie impaired hemostasis in mouse models of MYH9-related disease
Science Advances 8, 20 (2022)

K. Aurich, J. Wesche, M. Ulbricht, O. Otto, A. Greinacher, and R. Palankar
Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates
Scientific Reports 12, 6229 (2022)

L. Sachs, J. Wesche, L. Lenkeit, A. Greinacher, M. Bender, O. Otto, and R. Palankar
Ex vivo anticoagulants affect human blood biomechanics with implications for high-throughput functional mechanophenotyping
Communications Biology 5, 86 (2022)

R.H. Pires, T.H. Dau, E. Manu, N. Shree, and O. Otto
Switching in the expression pattern of actin isoforms marks the onset of contractility and distinct mechanodynamic behavior during cardiomyocyte differentiation
Physiological Reports 10, e15171 (2022)

A. Wilden, J. Glaubitz, O. Otto, D. Biedenweg, M. Nauck, M. Mack, S. Ribback, B. M. Bröker, S. F. von Rheinbaben, M. M. Lerch, A. A. Aghdassi, F. U. Weiss, and M. Sendler
Mobilization of CD11b+/Ly6chi monocytes causes multi organ dysfunction syndrome in acute pancreatitis
Front. Immunol. 13 (2022)

C.D. Much, B.S. Sandtner, K. Schwefel, E. Freund, S. Bekeschuss, O. Otto, A. Pagenstecher, U. Felbor, M. Rath, and S. Spiegler
Inactivation of cerebral cavernous malformation genes results in accumulation of von Willebrand factor and redistribution of Weibel-Palade bodies in endothelial cells
Frontiers in Molecular Biosciences 8, 622547 (2021)

K. Busch, M. Kny, N. Huag, T.E. Klassert, M. Stock, A. Hahn, S. Graeger, M. Todiras, S. Schmidt, B. Chamling, M. Willenbrock, S. Gross, D. Biedenweg, A. Heuser, C. Schneidereit, C. Butter, S.B. Felix, O. Otto, F.C. Luft, H. Slevogt, and J. Fielitz
Inhibition of the NLRP3/IL-1β axis protects against sepsis-induced cardiomyopathy
Journal of Cachexia, Sarcopenia and Muscle 12, 1653 – 1668 (2021)

C. Krüger, M. Waldeck-Weiermair, J. Kaynert, T. Pokrant, Y. Komaragiri, O. Otto, T. Michel, and M. Elsner
AQP8 is a crucial H2O2 transporter in insulin-producing RINm5F cells
Redox Biology 43, 101962 (2021)

S. Sinjari, J. S. Freitag, C. Herold, O. Otto, D. M. Smith, and H. D.H. Stöver
Tunable polymer microgel particles and their study using microscopy and real-time deformability cytometry
Journal of Polymer Science 58, 2317 – 2326 (2020)

T. Heida, O. Otto, D. Biedenweg, N. Hauck, and J. Thiele
Microfluidic Fabrication of Click Chemistry-Mediated Hyaluronic Acid Microgels: A Bottom-Up Material Guide to Tailor a Microgel’s Physicochemical and Mechanical Properties
Polymers 8, 1760 (2020)

I. Scheller, S. Stritt, S. Beck, B. Peng, I. Pleines, K. G. Heinze, A. Braun, O. Otto, R. Ahrends, A. Sickmann, M. Bender, and B. Nieswandt
Coactosin-like 1 integrates signaling critical for shear-dependent thrombus formation in mouse platelets
Haematologica 105, 1667 (2020)

M.H. Panhwar, F. Czerwinski, V.A.S. Dabbiru, Y. Komaragiri, B. Fregin, D. Biedenweg, R.H. Pires, and O. Otto
High-throughput cell and spheroid mechanics in virtual fluidic channels
Nature Communications 11, 2190 (2020)

K. Aurich, B. Fregin, R. Palankar, J. Wesche, O. Hartwich, D. Biedenweg, T.H. Nguyen, A. Greinacher, and O. Otto
Label-free on chip quality assessment of cellular blood products using real-time deformability cytometry
Lab on a Chip 20, 2306 – 2316 (2020)

M. Urbanska, H.E. Muñoz, J.S. Bagnall, O. Otto, S.R. Manalis, D. di Carlo, and J. Guck
A comparison of microfluidic methods for high-throughput cell deformability measurements
Nature Methods 17, 587 – 593 (2020)

E. Guzniczak, O. Otto, G. Whyte, T. Chandra, N. Robertson, N. Willoughby, M. Jiminez, and H. Bridle
Purifying stem cell-derived red blood cells: a high-throughput label-free downstream processing strategy based on microfluidic spiral inertial separation and membrane filtration
Biotechnology and Bioengineering 117:2032 – 2045 (2020)

E. Guzniczak, O. Otto, G. Whyte, N. Willoughby, M. Jiminez, and H. Bridle
Deformability-induced lift force in spiral microchannels for cell separation
Lab on a Chip 20:614 (2020)

R.H. Pires, N. Shree, E. Manu, E. Guzniczak, and O. Otto
Cardiomyocyte mechanodynamics under conditions of actin remodelling
Philosophical Transactions B 374, 20190081 (2019)

K. Schwefel, S. Spiegler, S. Ameling, C.D. Much, R.A. Pilz, O. Otto, U. Völker, U. Felbor, and M. Rath
Biallelic CCM3 mutations cause a clonogenic survival advantage and endothelial cell stiffening
Journal of Cellular and Molecular Medicine 23, 1771 – 1783 (2019)

B. Fregin, F. Czwerwinski, D. Biedenweg, Girardo, S., Gross S., K. Aurich, and O. Otto
Dynamic real-time deformability cytometry: high-throughput single-cell rheology in complex samples
Nature Communications 10, 415 (2019)

T. Santos-Ferreira, M. Herbig, O. Otto, M. Cardido, M.O. Karl, S. Michalakis, J. Guck, and M. Ader
Morpho-rheological fingerprinting of rod photoreceptors using real-time deformability cytometry
Cytometry A 23789 (2019)

S. Tietze, M. Kräter, A. Jacobi, A. Taubenberger, M. Herbig, R. Wehner, M. Schmitz, O. Otto, C. List, B. Kaya, M. Wobus, M. Bornhäuser, and J. Guck
Spheroid culture of mesenchymal stromal cells results in morpho-rheological properties appropriate for improved microcirculation
Advanced Science 6, 1802104 (2019)

M. Bender and O. Otto
No Escape- a novel tool to quantify platelet intra-thrombus movements
Thrombosis and Haemostasis 188, 1515 – 1516 (2019)

C. N. Holenstein, A. Horvvath, B. Schär, A. D. Schoenenberger, M. Bollhalder, N. Goedecke, G. Bartalena, O. Otto, M. Herbig, J. Guck, D. A. Müller, J. G. Snedeker, and U. Silvan
The relationship between metastatic potential and in vitro mechanical properties of osteosarcoma cells
Molecular Biology of the Cell, 30, 7 (2019)

E. Guzniczak, M. Jimenez, M. Irwin, O. Otto, N. Willoughby, and H. Bridle
Impact of poloxamer 188 (Pluronic F-68) additive on cell mechanical properties, quantification by real-time deformability cytometry
Biomicrofluidics 12, 044118 (2018)

M. Herbig, A. Mietke, P. Müller, and O. Otto
Statistics for real-time deformability cytometry: clustering, dimensionality reduction and significance testing
Biomicrofluidics 12, 042214 (2018)

P. Rosendahl, K. Plak, A. Jacobi, M. Kräter, N. Töpfner, O. Otto, C. Herold, M. Winzi, M. Herbig, Y. Ge, S. Girardo, K. Wagner, B. Baum, and J. Guck
Real-time fluorescence and deformability cytometry – flow cytometry goes mechanics
Nature Methods 15, 355-358 (2018)

N. Töpfner, C. Herold, O. Otto, P. Rosendahl, A. Jacobi, M. Kräter, J. Stächele, L. Menschner, M. Herbig, L. Ciuffreda, L. Ranford-Cartwright, M. Grzybek, Ü. Coskun, E. Reithuber, G. Gariss, P. Mellroth, B. Henriques-Normark, N. Tregay, M. Suttorp, M. Bornhäuser, E. R. Chilvers, R. Berner, and J. Guck
Detection of human disease conditions by single-cell morpho-rheological phenotyping of blood
eLife 7, 1-22 (2018)

S. Golfier, P. Rosendahl, A. Mietke, M. Herbig, J. Guck, and O. Otto
High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications
Cytoskeleton 74, 283-296 (2017)

S. Bekeschus, K. Rödder, B. Fregin, O. Otto, M. Lippert, K.D. Weltmann, K. Wende, A. Schmidt, and R.K. Gandhirajan
Toxicity and immunogenicity in murine melanoma following exposure to physical plasma- derived oxidants
Oxidative Medicine and Cellular Longevity 4396467 (2017)

M. Kräter, A. Jacobi, O. Otto, S. Tietze, K. Müller, D.M. Poitz, S. Palm, V.M. Zinna, U. Biehain, M. Wobus, T. Chavakis, C. Werner, J. Guck, and M. Bornhäuser
Bone marrow niche-mimetics modulate HSPC function via integrin signaling
Scientific Reports 7(1) 2549 (2017)

M. Koch, K.E. Wright, O. Otto, M. Herbig, N.D. Salinas, N.H. Tolia, T.J. Satchwell, J. Guck, N.J. Brooks, and J. Baum
Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion
PNAS 114, 201602843 (2017)

S. Ciucci, Y. Ge, C. Durán, A. Palladini, V. Jiménez-Jiménez, L.M. Martinés-Sánchez, Y. Wang, S. Sales, A. Shevchenko, S.W. Poser, M. Herbig, O. Otto, A. Androutsellis-Theotokis, J. Guck, M.J. Gerl, and C.V. Cannistraci
Enlightening discriminative network functional modules behind principal component analysis separation in differential-omic science studies
Scientific Reports 411, 408-421 (2017)

M. Mokbel, D. Mokbel, A. Mietke, N. Träber, S. Girardo, O. Otto, J. Guck, and S. Aland
Numerical simulation of real-time deformability cytometry to extract cell mechanical properties
ACS Biomaterials Science & Engineering 3, 2962-2973 (2017)

M. Xavier, P. Rosendahl, M. Herbig, M. Kräter, D. Spencer, M. Bornhäuser, R.O.C. Oreffo, H. Morgan, J. Guck, and O.Otto
Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry
Integrative Biology 8, 616 (2016)

M.C. Munder, D. Midtvedt, T. Franzmann, E. Nüske, O. Otto, M. Herbig, E. Ulbricht, P. Müller, A. Taubenberger, S. Maharana, L. Malinovska, D. Richter, J. Guck, V. Zaburdaev and S. Alberti
A pH-driven transition of the cytoplasm from a fluid – to a solid-like state promotes entry into dormancy
eLife 5, e09347 (2016)

H. Mziaut, B. Mulligan, P. Hoboth, O. Otto, A. Ivanova, M. Herbig, D. Schumann, T. Hildebrandt, J. Deghny, A. Sönmez, C. Münster, M. Meyer-Hermann, J. Guck, Y. Kalaidzidis, and M. Solimena
The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512
Molecular Metabolism 5 (8), 656-668 (2016)

A. Mietke, O. Otto, S. Girardo, P. Rosendahl, A. Taubenberger, S. Golfier, E. Ulbricht, S. Aland, J. Guck, and E. Fischer-Friedrich
Extracting cell stiffness from Real-Time Deformability Cytometry – a theoretical and experimental analysis
Biophysical Journal 109, 2023-2036 (2015)

C.J. Chan, A. E. Ekpenyong, S. Golfier, W. Li, K.J. Chalut, O. Otto, J. Elgeti, J. Guck, and F. Lautenschläger
Myosin II activity softens cells in suspension
Biophysical Journal 108, 1856-69 (2015)

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U.F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck
Real-time deformability cytometry: on-the-fly cell mechanical phenotyping
Nature Methods 12, 199-202 (2015)

A. Huhle, D. Klaue, H. Brutzer, P. Dahldrop, S. Joo, O. Otto, U.F. Keyser, and R. Seidel
Camera-based three-dimensional real-time particle tracking at kHz rates and Angström accuracy
Nature Communications 6, 5885 (2015)

M. Kreysing, D. Ott, M.J. Schmidberger, O. Otto, M. Schürmann, E. Martin-Badosa, G. Whyte, and J. Guck
Dynamic operation of optical fibres beyond the single-mode regime facilitates the orientation of biological cells
Nature Communications 5, 5481 (2014)

N. Laohakunakorn, S. Ghosal, O. Otto, K. Misiunas, and U.F. Keyser
DNA interactions in crowded nanopores
Nano Letters 13, 2798-2802 (2013)

N. Laohakunakorn, O. Otto, S. Sturm, K. Kroy, and U.F. Keyser
Dynamic single-molecule force spectroscopy using optical tweezers and nanopores
Conference: SPIE NanoScience + Engineering 12, 8810 (2013)

O. Otto, S. Sturm, N. Laohakunakorn, U.F. Keyser, and K. Kroy
Rapid internal contraction boosts DNA friction
Nature Communications 4, 1780 (2013)

N. Laohakunakorn, S. Ghosal, K. Misiunas, O. Otto, and U.F. Keyser
Electrophoretic force on multiple DNA molecules in a nanopore
Biophysical Journal 104, 517a (2013)

V. Fodera, S. Pagliara, O. Otto, U.F. Keyser, and A.M. Donald
Microfluidics reveals a flow-induced large-scale polymorphism of protein aggregates
Journal of Physical Chemistry Letters 3, 2803-2807 (2012)

L.J. Steinbock, A. Lucas, O. Otto, and U.F. Keyser
Voltage-driven transport of ions and DNA through nanocapillaries
Electrophoresis 33, 3480-8 (2012)

O.J. Pambos, K. Göpfrich, K.R. Mahendran, J.L. Gornall, O. Otto, L. Steinbock, C. Chimerel, M. Winterhalter, and U.F. Keyser
Towards simultaneous force and resistive pulse sensing in protein nanopores using optical tweezers
Proceedings of the International Conference Nanopores for Bioanalytical Applications (2012)

O. Otto, L. Steinbock, D. W. Wong, J. L. Gornall, and U.F. Keyser
Direct force and ionic-current measurements on DNA in a nanocapillary
Review of Scientific Instruments 82, 086102 (2011)

O. Otto, L. Steinbock, and U.F. Keyser
Nanocapillaries and optical tweezers for studies on DNA in confinement
SPIE Proceedings 8097 (2011)

J.L. Gornall, K.R. Mahendran, O.J. Pambos, L.J. Steinbock, O. Otto, C. Chimerel, M. Winterhalter, and U.F. Keyser
Simple reconstitution of protein pores in nano lipid bilayers
Nano Letters 11, 3334-3340 (2011)

C. Gutsche, M.M. Elmahdy, K. Kegler, I. Semenov, T. Stangner, O. Otto, O. Oeberschär, U.F. Keyser, M. Krueger, M. Rauscher, R. Weeber, J. Harting, W.W. Kim, V. Lobaskin, R.R. Netz, and F. Kremer
Micro-rheology on (polymer-grafted) colloids using optical tweezers
Journal of Physics: Condensed Matter 11, 184114 (2011)

O. Otto, J.L. Gornall, G. Stober, F. Czerwinski, R. Seidel, and U.F. Keyser
High-speed video-based tracking of optically trapped colloids
Journal of Optics 13, 044011 (2011)

O. Otto, A. Hueffner, L. Steinbock, J. Gonall, M. Stewart, and U.F. Keyser
Single molecule studies of nuclear transport
Biophysical Journal (Conference Contribution) 3, p249a (2011)

L.J. Steinbock, O. Otto, D. R. Skarstam, S. Jahn, C. Chimerel, J.L. Gornall, and U.F. Keyser
Probing DNA with micro- and nanocapillaries and optical tweezers
Journal of Physics: Condensed Matter 22, 454113 (2010)

O. Otto, F. Czerwinski, J. L. Gornall, G. Stober, L. B. Oddershede, R. Seidel, and U.F. Keyser
Real-time particle tracking at 10,000 fps using optical fiber illumination
Optics Express 18, 22722 – 22733 (2010)

L.J. Steinbock, O. Otto, C. Chimerel, J.L. Gornall, and U.F. Keyser
Detecting DNA folding with nanocapillaries
Nano Letters 10, 2493-2497 (2010)

I. Semenov, O. Otto, G. Stober, P. Papdopoulos, U.F. Keyser, and F. Kremer
Single colloid electrophoresis
Journal of Colloid and Interface Science 337, 260-264 (2009)

O. Otto, C. Gutsche, F. Kremer, and U.F. Keyser
Optical tweezers with 2.5 kHz bandwidth video detection for single-colloid-electrophoresis
Review of Scientific Instruments 83, 129901 (2008)