2022

52  Interpretation of cell mechanical experiments in microfluidic systems depend on the choice of cellular shape descriptors

Fregin B, Biedenweg D, Otto O; Biomicrofluidics 2022 Apr 28; 16(024109), doi: 10.1063/5.0084673

49 Ex vivo anticoagulants affect human blood platelet biomechanics with implications for higt-throughput functional mechanophenotyping

Sachs L, Wesche J, Lenkeit I, Greinacher A, Bender M, Otto O, Palankar R; Commun Biol. 2022 Jan 21; 5(1):86; doi:10.1038/s42003-021-02982-6

48 Machine learning assisted real-time deformability sytometry of CD34+cells allows to identify patients with myelodysplastic syndroms

Herbig M, Jacobi A, Wobus M, Weidner H, Mies A, Kräter M, Otto O, Thiede C, Weickert MT, Götze KS, Rauner M, Hofbauer LC, Bornhäuser M, Guck J, Ader M, Platzbecker U. Balian E.; Sci Rep. 2022 Jan 18; 12(1):870. doi:10.1038/841598-022-04939-z

2021

47 Inhibition of the NLRP3/IL-1ß axis protects against sepsis-induced cardiomyopathy

Busch K, Kny M, Huang N, Klassert TE, Stock M, Hahn A, Graeger S, Todiras M, Schmidt S, Chamling B, Willenbrock M, Groß S, Biedenweg D, Heuser A, Scheidereit C, Butter C, Felix SB, Otto O, Luft FC, Slevogt H, Fielitz J, Journal of Cachexia, Sacropenia and Muscle 2021 Dec; 12(6)_1653-1668; doi: 10.1002/jcsm.12763

46 Inactivation of Cerebral Cavernous Malformation Genes Results in Accumulation of von Willebrand Factor and Redistribution of Weibel-Palade Bodies in Endothelial Cells

Much CD, Sendtner BS, Schwefel K, Freund E, Bekeschus S, Otto O, Pagenstecher A, Felbor U, Rath M, Spiegler S; Front Mol Biosi. 2021 Jul 9; 8:622547; doi: 10.3389/fmolb.2021.622547

45 AQP8 is a crucial H2O2 transporter in insulin-producing RINm5F cells

Krüger C, Waldeck-Weiermair M, Kaynert J, Pokrant T, Komaragiri Y, Otto O, Michel T, Elsner M.; Redox Biol. 2021 Jul; 43:101962; doi: 10.1016/j.redox.2021.101962.

2020

44 Microfluidic Fabrication of Click Chemistry-Mediated Hyaluronic Acid Microgels: A Bottom-Up Material Guide to Tailor a Microgel´s Physiochemical and Mechanical Properties

Heida T, Otto O, Biedenweg D, Hauck N, Thiele J; Polymers (Basel) 2020 Aug 6; 12(8):1760. doi: 10.3390/polym12081760

43 Label-free on chip quality assessment of cellular blood products using real-time deformability cytemetry

Aurich K, Fregin B, Palankar R, Wesche J, Hartwich O, Biedenweg D, Nguyen TH, Greinacher A, Otto O; Lab Chip. 2020 Jun30; 20(13):2306. doi:10.1039/dolcoo258e

42 High-throughput cell and spheroid mechanics in virtual fluidic channels

Panhwar MH, Czerwinski F, Dabbiru VAS, Komaragiri Y, Fregin B, Biedenweg D, Nestler P, Pires RH, Otto O; Nat Commun 2020 May 04; doi:10.1038/s41467-020-15813-9, Online ahead of print

41 A comparison of microfluidic methods for high-throughput cell deformability measurements

Urbanska M, Muñoz HE, Shaw Bagnall J, Otto O, Manalis SR, Di Carlo D, Guck J; Nat Methods. 2020 Apr 27; doi:10.1038/s41592-020-0818-8; online ahead of print

40 Purifying stem cell-derived red blood cells: a high-throughput lable-free downstream processing strategy based on microfluidic spiral inetial separation and membrane filtration

Guzniczak E, Otto O, Whyte G, Chandra T, Robertson NA, Willoughby N, Jimenez M, Bridle H; Biotechnol Bioeng. 2020 FEb 26; doi:10.1002/bit.27319; online ahead of print

39 Deformability-induced Lift Force in Spiral Microchannels for Cell Separation

Guzniczak E, Otto O, Whyte G, Willoughby N, Jimenez M, Bridle H; Lab Chip. 2020 Jan 9; doi: 10.1039/c9lc01000a

2019

38 Cardiomyocyte mechanodynamics under conditions of actin remodelling

Pires RH, Shree N, Manu E, Guzniczak E, Otto O; Philos Trans R Soc Lond B Biol Sci 2019 Nov 5; 374(1786); doi:10.1098/rstb.2019.0081

37 High-Throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry

Fregin B, Czerwinski F, Biedenweg D, Girardo S, Gross S, Aurich K, Otto O; Nat Commun 2019 Jan 24; 10(1):415; doi:10.1038/s41467-019-08370-3

36 Tafazzin-dependent cardiolipin composition in C6 glioma cells correlates with changes in mitochondrial and cellular functions, and cellular proliferation

Gürtler S, Wolke C, Otto O, Heise N, Scholz F, laporte A, Elsner M, Jöns A, Weinert S, Döring M, Jansing S, Gardemann A, Lendeckel U, Schild L; Biochim Biophys Acto Mol Cell Biol Lipids 2019 Jan9; 1864(4):452-465; doi: 10.1016/j.bbalip.2019.01.006

35 Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis

Sarem M, Otto O, Tanaka S, Shastri VP; Stem Cell Res Ther. 2019 Jan 10; 10(1):10; doi:10.1186/s13287-018-1103-y

2018

34 Impact of poloxamer 188 (Pluronic F-68) additive on cell mechanical properties, quantification by real-time deformability cytometry

Guzniczak E, Jimenz M, Irwin M, Otto O, Willoughby N, Bridle H, Biomicrofluidics 2018 Aug 22; 12(4):044118; doi: 10.1063/1.5040316

33 Biallelic CCM3 mutations cause a clonogenic survival advantage and endothelial call stiffening

Schwefel K, Spiegler S, Ameling S, Much CD, Pilz RA, Otto O, Völker U, Felbor U, Rath M; J Cell Mol Med. 2018 Dec 13; doi:10.1111/jcmm.14075

32 No Escape-A Novel Tool to Quantify Platelet Intra-Thrombus Movements

Bender M, Otto O; Thromb Haemost. 2018 Sept; 118(9):1515-1516; doi: 10.1055/s-0038-1668587

31 Statistics for real-time deformability cytometry: Clustering, dimensionality reduction, and significance testing

Herbig M, Mietke A, Müller P, Otto O; Biomicrofluidics 2018 Jun 4; 12(4):042214; doi: 10.1063/1.5027197

30 Real-time fluorescence and deformability cytometry

Rosendahl P, Plak K, Jacobi A, Kraeter M, Toepfner N, Otto O, Herold C, Winzi M, Herbig M, Ge Y, Girardo S, Wagner K, Baum B, Guck J; Nat Methods 2018 May; 15(5):355-358; doi: 10.1038/nmeth.4639

29 Detection of human diseas conditions by single-cell morpho-rheological phenotyping of blood

Toepfner N, Herold C, Otto O, Rosendahl P, Jacobi A, Kräter M, Stächele J, Menschner L, Herbig M, Ciuffreda L, Ranford-Cartwright L, Grzybek M, Coskun Ü, Reithuber E, Garriss G, Mellroth P, Henriques-Normark B, Tregay N, Suttorp M, Bornhäuser M, Chilvers ER, Berner R, Guck J; Elife 2018 Jan 13; 7.pii:e29213; doi: 10.7554/eLife.29213

28 Real-time Deformability Cytometry: Label-Free Functional Characterization of Cells

Herbig M, Kräter M, Plak K, Müller P, Guck J, Otto O; Methods Mol Biol. 2018 ; 1678:347-369; doi: 10.1007T

2017

27 Toxicity and Immunogenicity in Murine Melanoma following Exposure to Physical Plasma-Derived Oxidants

Bekeschus S, Rödder K, Fregin B, Otto O, Lippert M, Weltmann KD, Wende K, Schmidt A, Gandhirajan RK; Oxid Med Cell Longev. 2017; 2017:4396467; doi: 10.1155/2017/4396467

26 Bone marrow niche-memetics modulate HSPC function via integrin signaling

Kräter M, Jacobi A, Otto O, Tietze S, Müller K, Poitz DM, Palm S, Zinna VM, Biehain U, Wobus M, Chavakis T, Werner C, Guck J, Bornhauser M; Sci Rep. 2017 May 31; 7(1):2549; doi: 10.1038/s41598-017-02352-5

25  Actin stress fiber orgnazation promotes cell stiffening and proliferation of pre-invasive breast cancer cells

Tavares S, Vieira AF, Taubenberger AV, Araújo M, Martines NP, Brás-Pereira C, Polónia A, Herbig M, Barreto C, Otto O, Cardoso J, Pereira-Leal JB, Guck J, Paredes J, Janody F; Nat Commun. 2017 May 16; 8:15237; doi: 10.1038/ncomms15237

24 High-throughput cell mechanical phentotyping for label-free titration assays of cytoskeletal modifaications

Golfier S, Rosendahl P, Mietke A, Herbig M, Guck J, Otto O; Cytoskeleton (Hoboken) 2017 Aug; 74(8):283-296; doi: 10.1002/cm.21369

23 Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion

Koch M, Wright KE, Otto O, Herbig M, Salinas ND, Tolia NH, Satchwell TJ, Guck J, Brooks NJ, Baum J, Proc Natl Acad Sci USA 2017 Apr 18; 114(16).4225-4230; doi: 10.1073/pnas.1620843114

22 Enlightening discriminative network functional modules behind Principal Component Analysis separation in differential-omic science studies

Ciucci S, Ge Y, Durán C, Palladini A, Jiménez-Jiménez V, Martinéz-Sánchez LM, Wang Y, Sales S, Shevchenko A, Poser SW, Herbig M, Otto O, Androutsellis-Theotokis A, Guck J, Gerl MH, Cannistraci CV; Sci Rep. 2017 Mar 13; 7:43946; doi: 10.1038/srep43946

2016

21 The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512

Mziaut H, Mulligan B, Hoboth , Otto O, Ivanova A, Herbig M, Schumann D, Hildebrandt T, Dehghany J, Sönmez A, Münster C, Meyer-Hermann M, guck J, Kalaidzidis Y, Solimena M; Mol Metab. 2016 Jun 10; 5(8);656-668; doi: 10.1016/molmet.2016.05.015

20 A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy

Munder MC, Midtvedt D, Franzmann T, Nüske E, Otto O, Herbig M, Ulbricht E, Müller P, Taubenberger A, Maharana S, Malinovska L, Richter D, Guck J, Zaburdaev V, Alberti S; Elife 2016 Mar 22; 5. pii: e09347; doi: 10.7554/eLife.09347

19 Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry

Xavier M, Rosendahl P, Herbig M, Kräter M, Spencer D, Bornhäuser M, Oreffo RO, Morgan H, Guck J, Otto O; Intergr Biol (Camb). 2016 May 16; 8(5):616-23; doi: 10.1039/c5ib00304k

2015

18 Real-time deformabilty cytometry as a label-free indicator of cell function

Otto O, Rosendahl P, Golfier S, Mietke A, Herbig M, Jacobi A, Topfner N, Herold C, Klaue D, Girardo S, Winzi M, Fischer-Friedrich E, Guck J; Conf Proc IEEE Eng Med Biol Soc. 2015; 2015:1861-4; doi: 10.1109/EMBC.2015.7318744

17 Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment

Mietke A, Otto O, Girardo S, Rosendahl P, Taubenberger A, Golfier S, Ulbricht E, Aland S, Guck J, Fischer-Friedrich E; Biophys J. 2015 Nov 17; 109(10):2023-36; doi: 10.1016/j.bpj.2015.09.006

16 Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia

Wobus M, Bornhäuser M, Jacobi A, Kräter M, Otto O, Ortlepp C, Guck J, Ebninger G, Thiede C, Oelschlägel U; Oncotarget. 2015 Nov 17; 6(36):38804-15; doi: 10.18632/oncotarget.5661

15 Myosin II Activity Softens Cells in Suspension

Chan CJ, Ekpenyong AE, Golfier S, Li W, Chalut KJ, Otto O, Elgeti J, Guck J, Lautenschläger F, Biophys J, 2015 Apr 21; 08(8):1856-69; doi: 10.1016/j.bpj.2015.03.009

14 Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

Otto O, Rosendahl P, Mietke A, Golfier S, Herold C, Klaue D, Girardo S, Pagliara S, Ekpenyong A, Jacobi A, Wobus M, Töpfner N, Keyser UF, Mansfeld J, Fischer-Friedrich E, Guck J; Nat Methods. 2015 Mar; 12(3):199-202; 4 p following 202; doi: 10.1038/nmeth.3281

13 Camera-based three-dimensional real-time particle tracking at kHz rates and Ångström accuracy

Huhle A, Klaue D, Brutzer H, Daldrop P, Joo S, Otto O, Keyser UF, Seidel R; Nat Commun. 2015 Jan 7; 6:5885; doi: 10.1038/ncomms6885

2014

12 Dynamic operation of optical fibres beyond the single-mode regime facilitates the orientation of biological cells

Kreysing M, Ott D, Schmidberger MJ, Otto O, Schürmann M, Martin-Badosa E, Whyte G, Guck J; Nat Commun. 2014 Nov 20; 5:5481; doi: 10.1038/ncomms6481

2013

11 Rapid internal contraction boosts DNA friction

Otto O, Sturm S, Laohakunakorn N, Keyser UF, Kroy K; Nat Commun. 2013; 4:1780; doi: 10.1038/ncomms2790

10 DNA interactions in crowded nanopores

Laohakunakorn N, Ghosal S, Otto O, Misiunas K, Keyser UF; Nano Lett. 2013 Jun 12; 13(6):2798-802; doi: 10.1021/nl401050m

2012

09 Voltage-driven transport of ions and DNA through nanocapillaries

Steinbock LJ, Lucas A, Otto O, Keyser UF; Electrophoresis. 2012 Dec; 33(23):3480-7; doi: 10.1002/elps.201100663

2011

08 Note: Direct force and ionic-current measurements on DNA in a nanocapillary

Otto O, Steinbock LJ, Wong DW, Gornall JL, Keyser UF; Rev Sci Instrum. 2011 Aug; 82(8):086102; doi: 10.1063/1.3623502

07 Simple reconstitution of protein pores in nano lipid bilayers

Gronall JL, Mahendran KR, Pambos OJ, Steinbock LJ, Otto O, Chimerel C, Winterhalter M, Keyser UF; Nano Lett. 2011 Aug 10; 11(8):3334-40; dio: 10.1021/nl201707d

06 Micro-rheology on (polymer-grafted) colloids using optical tweezers

Gutsche C, Elmahdy MM, Kregler K, Semenov I, Stangner T, Otto O, Ueberschär O, Keyser UF, Krueger M, Rauscher M, Weeber R, Harting J, Kim YW, Lobaskin V, Netz RR, Kremer F; J Phys Condens Matter. 2011 May 11; 23(18):184114; doi: 10.1088/0953-8984/23/18/184114

2010

05 Probing DNA with micro- and nanocapillaries and optical tweezers

Steinbock LJ, Otto O, Skarstam DR, Jahn S, Chimerel C, Cornall JL, Keyser UF; J Phys Condens Matter. 2010 Nov 17; 22(45):454113; doi: 10.1088/0953-8984/22/45/454113

04 Real-time particle tracking at 10,000 fps using optical fiber illumination

Otto O, Czerwinski F, Gornall JL, Stober G, Oddershede LB, Seidel R, Keyser UF; Opt Express. 2010 Oct 25; 18(22):22722-33; doi: 10.1364/OE.18.022722

03 Detecting DNA folding with nanocapillaries

Steinbock LJ, Otto O, Chimerel C, Gornall J, Keyser UF; Nano Lett. 2010 Jul 14; (7):2493-7; doi: 10.1021/nl100997s. Erratum in: Nano Lett. 2013 Jul 10; 13(7):3444

2009

02 Single colloid electrophoresis

Semenov I, Otto O, Stober G, Papadopoulos P, Keyser UF, Kremer F; J Colloid Interface Sci. 2009 Sep 1; 337(1):260-4; doi: 10.1016/j.jcis.2009.05.017

2008

01 Optical tweezers with 2.5 kHz bandwidth video detection for single-colloid electrophoresis

Otto O, Gutsche C, Kremer F, Keyser UF; Rev Sci Instrum. 2008 Feb; 79 (2 Pt 1):023710; doi: 10.1063/1.2884147